Primer Sequences: Unlocking the Secrets of DNA Amplification

What are five examples of primers, their target organisms, and their primer sequences?

Below are five examples of primers, their target organisms, and their primer sequences:

1. Organism: Escherichia coli (Bacteria)
Forward Primer: 5'-TACGGGAGGCAGCAG-3'
Reverse Primer: 5'-CGATTACTAGCGATTTC-3'
Target: These primers amplify a conserved region in the 16S rRNA gene, commonly used for bacterial identification.

2. Organism: Homo sapiens (Human)
Forward Primer: 5'-AGGTGAAGGTCGGAGTCA-3'
Reverse Primer: 5'-CTGCTCAGGAGGAGCAAT-3'
Target: These primers amplify a specific gene region, such as a disease-related gene, for diagnostic or research purposes.

3. Organism: Arabidopsis thaliana (Plant)
Forward Primer: 5'-TGTCTTCGAGGCCATTTGA-3'
Reverse Primer: 5'-ACACACCTCGTAGCCATAC-3'
Target: These primers amplify a gene related to plant development or stress response.

4. Organism: Saccharomyces cerevisiae (Yeast)
Forward Primer: 5'-GGTTTCATCAGCTGCTC-3'
Reverse Primer: 5'-CGAAGTAGTGGTCGTGGA-3'
Target: These primers amplify a region of a gene involved in yeast metabolism or fermentation.

5. Organism: Plasmodium falciparum (Malaria Parasite)
Forward Primer: 5'-ATGGAGGATAATTATATC-3'
Reverse Primer: 5'-TAAAGTAGAAACTACGCT-3'
Target: These primers amplify a specific region of the parasite's DNA for malaria diagnosis.

These carefully designed primer sets ensure specificity and efficiency in amplifying the target DNA, supporting various research, diagnostic, and biotechnological applications without causing non-specific amplification.